Device

Part:BBa_I746202

Designed by: Narin Hengrung   Group: iGEM07_Cambridge   (2007-08-31)

Outer Membrane Permeability Device

This device employs the FepA L8T mutant described here to allow molecules larger than normal to pass through the outer membrane of E. coli. The porins usually present will allow molecules up to around 700Da to freely diffuse in and out, however with this device, molecules of at least 1400Da should be able to pass through. Permeability was tested using the antibiotic Bacitracin. Wild type E. coli are resistant to this as the molecule is 1422Da in size. Expressing the device made the bacteria sensitive to bacitracin. In this device, the mutant FepA is under the control of a very high strength promotor.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 299
    Illegal AgeI site found at 665
    Illegal AgeI site found at 1292
    Illegal AgeI site found at 1810
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None